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This contrasts with the traditional view of the five kingdoms of life: Bacteria (or Monera), 50(2):171-184, 2002. 7 amol of carbonic anhydrase in a crude extract from human blood [152], Biophysics of DNA-Protein Interactions, 107 Biological and Medical Physics, Biomedical Engineering, DOI 10. Method and information obtained Solubility (distinction between (13)-β-D-glucan and cellulose; however, this method is not completely reliable) Infrared spectroscopy (identification of linkage typestructure) Methylation (gas chromato- graphy coupled to mass spectrometry) (identification of linkage type and estimation of the degree of polymerization if 100) 13C-NMR spectroscopy in DMSO (or NaOH) solution (structure) Expected results for in vitro (13)-β-D-glucans Insoluble in water Usually soluble in DMSO Usually soluble in 1 M NaOH Absorption band at 889.

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