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Repeating the calculations of Example 12-23 with the volume twenty times higher (V 10. viii CONTENTS Organelles Bounded by Single-Membrane Envelopes, 58 Peroxisomes, 59 Endoplasmic Reticulum, 60 Golgi Apparatus, 60 Lysosomes, 61 Summary, 61 Review Questions, 62 Answers to Review Questions, 63 4 DNA STRUCTURE AND THE GENETIC CODE, 65 Introduction, 65 The Structure of DNA, 65 The DNA Molecule Is a Double Helix, 68 The Two DNA Chains Are Complementary, 69 Different Forms of DNA, 71 DNA as the Genetic Material, 71 Packaging of DNA Molecules into Chromosomes, 71 Eukaryotic Chromosomes and Chromatin Structure, 71 Prokaryotic Chromosomes, 73 Plasmids, 74 Viruses, 74 The Genetic Code, 75 Amino Acid Names Are Abbreviated, 79 The Code Is Degenerate But Unambiguous, 79 Start and Stop Codons and the Reading Frame, 79 The Code Is Nearly Universal, 80 Missense Mutations, 80 Summary, 81 Further Reading, 84 Review Questions, 84 Answers to Review Questions, 85 5 DNA AS A DATA STORAGE MEDIUM, 87 Introduction, 87 DNA Replication, 87 The DNA Replication Fork, 88 Proteins Open up the DNA Double Helix During Replication, 88 DnaA Protein, 88 DnaB and DnaC Proteins, 90 Single-Strand Binding Proteins, 90 Biochemistry of DNA Replication, 90 DNA Synthesis Requires an RNA Primer, 90 RNA Primers Are Removed, 92 The Self-Correcting DNA Polymerase, 92 DNA Repair, 94 Spontaneous and Chemically Induced Base Changes, 94 Repair Processes, 94 Gene Structure and Organization in Eukaryotes, 98 Introns and Exons-Additional Complexity in Eukaryotic Genes, 98 The Major Classes of Eukaryotic DNA, 99 Gene Nomenclature, 101 Summary, 101 Further Reading, 102 Review Questions, 102 Answers to Review Questions, 103 6 TRANSCRIPTION AND THE CONTROL OF GENE EXPRESSION, 105 Structure of RNA, 105 RNA Polymerase, 106 Gene Notation, 106 Bacterial RNA Synthesis, 106 Control of Bacterial Gene Expression, 109 lac, an Inducible Operon, 111 trp, a Repressible Operon, 116 Eukaryotic RNA Synthesis, 118 Messenger RNA Processing, 118 Control of Eukaryotic Gene Expression, 119 Benefits of Predictive Maintenance 71 on the survey results, major improvements can be achieved in maintenance costs, unscheduled machine failures, repair downtime, spare parts inventory, and both direct and indirect overtime premiums.

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